Philipp. Sci. Lett. 2014 7 (2) 331-336
available online: October 12, 2014
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Received: December 11, 2013
Revised: June 5, 2014
Accepted: July 31, 2014
In vitro culture of myxomycetes offers potential application for the mass production of their natural products. We grew in vitro 18 species of myxomycetes on a modified, solid, semi-defined medium (SDM) for up to 4 weeks. Of these, 10 developed into amoeboflagellates after germination on diluted semidefined medium (dSDMA) and/or sterile water, while 7 grew into plasmodia. The amoeboflagellates were preserved in 15% glycerol and stored for 3 months at 5oC. Revival of the preserved amoeboflagellates resulted in a 90% success rate. This is the first attempt to store and preserve amoeboflagellates of myxomycetes. In vitro-grown plasmodia of P. compressum and plasmodia derived from sclerotia of unidentified myxomycetes were also tested for their ability to produce extracellular enzymes. Results showed the species excreted amylase and protease. This finding suggests an alternative mode of nutrition for myxomycetes in addition to the phagotrophic mode of nutrition known for these organisms.