ISSN 2094-2818

Editors: Eduardo A. Padlan and
Gisela P. Padilla-Concepcion
VOLUME 7 NUMBER 2 (July to December 2014)
 

Phil. Sci. Lett. 2014 7 (2) 438-444
available online: December 26, 2014

*Corresponding author
Email Address: cruz.merlyn@auf.edu.ph
Submitted: November 13, 2014
Revised: December 12, 2014
Accepted: December 12, 2014
Published: December 26, 2014
Guest Editor: Rosario G. Monsalud

Keywords: Escherichia coli, antibiotic resistance, beta-lactamase, extended-spectrum beta-lactamase, double-disk synergy test

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  ARTICLE
Evaluation of extended-spectrum beta-lactamase production in Escherichia coli clinical isolates from three hospitals in Luzon, Philippines

Merlyn C. Cruz*1,2, Catherine S. Bacani1, Alma B. Mendoza1, and Cynthia T. Hedreyda2

1Angeles University Foundation, Angeles City
2National Institute of Molecular Biology and Biotechnology, University of the Philippines, Diliman Quezon City

The increased spread of pathogens producing extended-spectrum beta-lactamase (ESBL) enzyme among members of family Enterobacteriaceae has been a serious problem worldwide. The absence of a fast and accurate detection protocol for ESBL producers had contributed to treatment failures in ESBL-associated infections. This study focused on the isolation of phenotypically ESBL-producing E. coli isolates from 3 hospitals in Luzon, Philippines. The availability of confirmed ESBLs is valuable in conducting experiments to gain information about ESBLs that are relevant to the identification of detection markers for these pathogens. Out of 119 pathogenic E. coli isolates from clinical samples such as urine, wound, body aspirates and blood, 37 (31%) were identified as suspected ESBLs using a preliminary test and 27 of the 37 suspected ESBLs were confirmed as ESBL producers based on a test referred to as double-disk synergy test. The highest percentage of ESBL-producing E. coli was obtained from urine specimens, while the lowest was from blood samples. All the ESBL-positive isolates exhibited resistance to cefotaxime and to levofloxacin. Co-resistance to other antibiotics like trimethoprim-sulfamethoxazole, ciprofloxacin, tetracycline and cefepime was also noted in some isolates. Susceptibility of ESBL-producers remains high towards carbapenems. These findings point to the urgent need to develop a fast and accurate detection protocol (including a molecular approach) for ESBL-producing pathogens.