Philippine Science Letters
vol. 6 | no. 2 | 2013
published online July 19, 2013


Antimicrobial and Antioxidant Activities of Fungal Leaf Endophytes Associated with Pandanus amaryllifolius Roxb.

by Melfei E. Bungihan1,6*, Maribel G. Nonato1,2,3, Siegfried Draeger4+, Scott Franzblau5 and Thomas Edison E. dela Cruz1,2,3

1 Graduate School,
2 College of Science and
3 Research Center for the Natural and Applied Sciences, University of Santo Tomas, España   1015 Manila, Philippines
4 Institute of Microbiology, University of Braunschweig, Spielmannstraße 7, D-38106   Braunschweig, Germany
5 Institute of Tuberculosis Research, College of Pharmacy, University of Illinois at Chicago,   Chicago, Illinois 60612-7231, USA
6 Center for the Natural Sciences, Saint Mary’s University, Bayombong, 3700 Nueva Vizcaya,   Philippines



Fungal endophytes are potential sources of novel and bioactive natural products. Our research study aimed to isolate, identify, and evaluate biological activities of endophytic fungi associated with the leaves of Pandanus amaryllifolius. Fungi were isolated after surface-sterilization of mature leaves of P. amaryllifolius collected from Nueva Vizcaya and Manila, Philippines. Twenty eight (28) morphospecies were isolated and identified as species belonging to the genera Colletotrichum, Chaetomium, Diaporthe, Glomerella, Guignardia, Lasiodiplodia, Lulworthia, Phoma, Phyllosticta, Trichoderma, and Truncatella. Selected fungal isolates were cultured in Potato Dextrose Broth for 3 weeks for the production of secondary metabolites. Extraction with ethyl acetate produced crude culture extracts which were observed to contain phenolics and sterols when subjected to thin layer chromatography. Bioassay of the crude extracts showed antimicrobial activities against Mycobacterium tuberculosis H37Rv using the Microplate Alamar Blue Assay method and against Staphylococcus aureus, Eschericia coli, and Gordonia terrae using the paper disk diffusion method. Crude culture extracts of Colletotrichum spp., Glomerella sp., and Chaetomium sp. showed the highest inhibitory activities against the test bacteria. Antioxidant activity using 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay also showed radical scavenging activity (16-37%) of the phenolic compounds from the crude culture extracts.

*Corresponding author
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Submitted :February 9, 2013
Revised: June 3, 2013
Accepted: June 5, 2013
Published: July 19, 2013
Editor-in-charge: Cynthia T. Hedreyda
Virginia C. Cuevas
Roland M. Hipol
Rosario G. Monsalud