Philippine Science Letters
vol. 6 | no. 2 | 2013
published online December 13, 2013


ARTICLE


Correlation of total IgE, house-dust mite specific IgE and absolute eosinophils in an asthmatic pediatric population


by Maricar W. Ching1,4*, Jennifer Maries G. Yap1,4, Kevin Carl P. Santos5, Cesar M. Ong6, and John Donnie A. Ramos1,2,3

1The Graduate School,
2Department of Biological Sciences, College of Science,
3Research Center for the Natural and Applied Sciences, University of Santo Tomas, Manila,
 Philippines;
4Biological Sciences Department, Centro Escolar University, Manila, Philippines;
5School of Statistics, University of the Philippines-Diliman, Quezon City, Philippines;
6Philippine Children Medical Center, Quezon City, Philippines





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The severity of asthma is influenced by multiple factors including absolute eosinophil count, total IgE and specific IgE resulting from sensitization to various allergens such as those from the house dust mite species, Blomia tropicalis (Bt), Dermatophagoides pteronyssinus (Dp), and Dermatophagoides farinae (Df). An observational-analytical, case-control method was used to examine 250 age- and sex-matched allergic asthma and non-asthmatic controls. Total serum IgE and HDM-specific IgE were determined using ELISA, while complete blood count using automated Coulter Counter was used to obtain the absolute eosinophil count. Log-transformed data were statistically analyzed for correlation. Significantly elevated levels of total IgE (p<0.0001), HDM-specific IgE (p<0.0001) and absolute eosinophil count (p<0.0001) were observed in allergic asthma cases compared to non-asthmatic controls. Patients with allergy, regardless of atopy status, and patients with allergic asthma concurrent with allergic rhinitis, showed significant correlation between Df-specific IgE and absolute eosinophil count (p=0.03). Moreover, significant correlation was observed between Dp-specific IgE and absolute eosinophil counts in allergic asthmatics concurrently with allergic rhinitis and atopic dermatitis (p=0.003). Multiple regression analyses showed that an increase in Dp-specific IgE will yield an expected increase in absolute eosinophil count holding other variables constant among allergic asthmatics, concurrently suffering with allergic rhinitis and atopic dermatitis (p=0.009). HDM-specific IgE, particularly Dp- and Df-specific IgE, is significantly correlated with and contributing to the absolute eosinophil count in allergic asthma among selected population of pediatric patients in the Philippines.


*Corresponding author
Email Address: cindywiscoching@gmail.com
Submitted: February 22, 2013
Revised: June 26, 2013
Accepted: August 30, 2013
Published: December 13, 2013
Editor-in-charge: Philip Ian P. Padilla